RecordsĬoordinates if one or more points have been defined using the point selection tool. Calculates line lengthĪnd angle if a line selection has been created using one of the three line selection tools. Has been selected using one of the first four tools in the tool bar. The image and click to the left of one of the drawn outlines with theīased on the selection type, calculates and displays either area statistics, line lengths and angles,Īrea statistics are calculated if there is no selection or if a subregion of the image Multiple selections can be saved by transferring them to a blank window, drawing each one using Edit/Draw,Īnd saving the resulting image. Selections can be saved to disk using File/Save As/ROI and restored using File/Open. Theshold Gray Levels that correspond towhat you want to measure: Image / Adjust / Theshold Pick the Gray scale image that best delimits the cells of interest Use Image / Color / Split Channels to make 3 gray Scale Images from your RGB image You edit the data in the spreadsheet to keep track of tissues, etc. Once you have collected all your measurements, click File / Save As/Īnd save the data as an excel sheet. Repeat for as many regions you want to measure. Select Straight Line, Drag and drop a line to correspond with region toīe measured.

are calculated from the values of the pixels along the line. Standard deviation, mode, min, max and bounding rectangle (v1.34l or later). With line selections, the following parameters can be recorded: length, angle (straight lines only), mean, Use the Analyze>Set Measurements command to specify what area statistics are recorded. ImageJ will have automatically filled in the Distance in Pixelsįield based on the length of the line selection. The known distance and unit of measurement, then click OK. Then, bring up the Set Scale dialog, enter Line selection tool to make a line selection that corresponds to Before using this command, use the straight Measurement results can be presented in calibrated units, Use this dialog to define the spatial scale of the active image so Brief Instructions for ImageJ Brief instructions for basic image analysis using ImageJ

Imagej threshold selection